The researcher attempted to find out how internship experiences vary with reference to professional development, career preparedness, and challenges faced during internship across two academic disciplines of Social Science and Humanities Stream and Science and Technology Stream. The researchers used a descriptive research design and collected data from 105 interns using convenience sampling in Mumbai city. The researcher found that professional skill development due to internship was rated higher by Social Science and Humanities stream in comparison to Science and Technology Stream. Career Preparedness outcomes were rated higher by Social Science and Humanities stream in comparison to Science and Technology Stream. For the challenges faced, Social Science & Humanities Stream perceive the issue of not receiving a stipend and balancing academics and internship responsibilities as a greater concern compared to those in Science & Technology Stream. Furthermore, for challenges faced, both the streams reported similar levels of difficulty in finding a suitable internship, perceived mentorship and guidance issues similarly, perceived workload intensity similarly, and reported comparable level of learning opportunities.