The present study was conducted to evaluate the allelopathic effects of stem and root leachates of Pluchea lanceolata on seed germination and seedling growth of two wheat varieties (UP 2338 and HUW 234). Different concentrations (5%, 10%, 15%, and 20%) of leachates Pluchea lanceolata were applied under laboratory conditions. Results revealed that increasing concentration of leachates significantly reduced germination percentage, shoot length, and root length. Root growth was found to be more sensitive than shoot growth. Root leachate exhibited stronger inhibitory effects compared to stem leachate. Among the two varieties, HUW 234 showed relatively higher tolerance than UP 2338. The study highlights the importance of allelopathy in crop–weed interactions.