[23] ENHANCING SALINITY RESILIENCE IN VIGNA RADIATA L. THROUGH BRASSINOLIDE AND Α-TOCOPHEROL-MEDIATED OXIDATIVE STRESS REGULATION

ARTICLE INFO: Date of Submission: Mar 4, 2026, Revised: Mar 12, 2026, Accepted: Mar 15 , 2026, CrossRef D.O.I : https://doi.org/10.56815/ijmrr.v5i3.2026.243-255. How To Cite: Aamir Abdullah, R. Somasundaram, K. Silambarasan (2026). Enhancing Salinity Resilience in Vigna radiata L. through Brassinolide and α-Tocopherol-Mediated Oxidative Stress Regulation. International Journal of Multidisciplinary Research & Reviews 5(3), 243-255.

Abstract

Salinity stress disrupts cellular redox homeostasis, leading to an imbalance in the production and scavenging of reactive oxygen species (ROS). This imbalance primarily arises from disturbances in the electron transport chains during photoinhibition and from reduced cellular water potential. Under saline conditions, ROS levels increase rapidly. Excessive accumulation of ROS can become cytotoxic, causing damage to cellular components and potentially leading to cell death if not effectively regulated. In the present investigation, a pot culture experiment was carried out to examine the effects of sodium chloride (NaCl) stress and the potential ameliorative role of the plant growth regulators brassinolide and α-tocopherol in Vigna radiata L. The growth regulators were administered through foliar spraying, with brassinolide applied at a concentration of 4 mg L⁻¹ and α-tocopherol at 200 mg L⁻¹, whereas salinity stress was imposed by soil drenching with 80 mM NaCl solution. The experimental plants were arranged into six treatment
groups: control, NaCl alone, NaCl combined with brassinolide, NaCl combined with α-tocopherol, brassinolide alone, and α-tocopherol
alone. Plant samples were collected randomly on the 25th, 35th, and 45th days after sowing for the assessment of various parameters. The results indicated that foliar supplementation with α-tocopherol and brassinolide significantly alleviated the adverse effects of salinity stress in Vigna radiata. This mitigation was evidenced by a reduction in oxidative stress indicators, particularly malondialdehyde (MDA) and hydrogen peroxide (H₂O₂), suggesting an improved oxidative balance and enhanced stress tolerance in treated plants.